www.medenosrce.net/arhimed   arhimed(a-t>medenosrce.net   [22/04/2026 01:26:34] http://www.medenosrce.net/arhimed/poglej.asp?id=100 Vpliv spola in spolnih hormonov na nastanek akutnih ishemicnih poškodb pri izoliranem podganjem srcu Avtor: Lea Andjelkovic, Jasna Šuštaršic Mentor: Metka V. Budihna, Gorazd Drevenšek Izhodišce: Spol je pomemben dejavnik pri pogostnosti nekaterih ishemicnih bolezni srca. Vpliv dodajanja spolnih hormonov poskusnim živalim na nastanek postishemicnih poškodb na srcu je samo delno raziskan. Namen: V naši nalogi smo želeli ugotoviti vpliv subkronicnega dajanja estradiola in testosterona podganam ter vpliv brejosti podgan na nastanek miokardnih okvar, povzrocenih z akutno ishemijo/reperfuzijo pri izoliranih srcih teh živali. Hipoteza: Spol in spolni hormoni vplivajo na delovanje srca, zato se bodo srca živali, ki smo jim dajali spolne hormone, na akutno ishemijo odzvala z manj poškodbami kot srca kontrolnih skupin. Metode: Poskuse smo izvajali na izoliranih srcih spolno zrelih podgan (230-330g) obeh spolov. Srca podgan smo izolirali po Langendorffu in jih perfundirali z raztopino Krebs-Henseleit, nasiceno s karbogenom. Srca smo 30 minut aerobno perfundirali in jih nato izpostavili 40 minutni globalni ishemiji, cemur je sledila 55 minutna reperfuzija. Za oceno stopnje okvare miokarda, povzrocene z ishemijo/reperfuzijo, smo uporabili naslednje merjene spremenljivke: pretok skozi koronarne arterije, stopnjo sprošcanja laktatne dehidrogenaze (LDH), tlak v levem prekatu, frekvenco utripov srca in pogostnost pojavljanja prekatnih fibrilacij v casu reperfuzije. Rezultati: Postishemicni koronarni pretok je bil višji pri srcih podgan, ki smo jim dajali estradiol oz. testosteron kot pri kontrolnih skupinah. Pri srcih samic z dodanim estradiolom je bil koronarni pretok znacilno višji (p < 0,05) kot pri srcih kontrolne skupine samic. Stopnja sprošcanja LDH je po ishemiji narasla pri vseh poskusnih skupinah. Najvišje vrednosti smo izmerili pri skupini samic po kotitvi, ki so bile znacilno višje kot pri samicah z dodanim estradiolom (p < 0,05). Frekvenca srcnih utripov je bila po ishemiji nižja kot pred ishemijo, najvišja je bila pri skupini samic po kotitvi. Trajanje fibrilacij je bilo pri skupinah z dodanima hormonoma krajše kot pri kontrolnih skupinah, vendar so bile razlike neznacilne. Zakljucek: Razlike v postishemicnih spremembah merjenih spremenljivk pri razlicnih poskusnih skupinah kažejo na zašcitno vlogo spolnih hormonov pri nastanku okvar na izoliranih podganjih srcih med ishemijo/reperfuzijo in potrjujejo našo hipotezo. «» [Abstract / English version] Subchronic pre-treatment of rats with sex hormones diminishes postishaemic damage of their isolated hearts Author: Lea Andjelkovic, Jasna Šuštaršic Mentor: Metka V. Budihna, Gorazd Drevenšek Background: Sex might be an important factor in the development of some ischaemic diseases of the heart. The influence of application of sex hormones to experimental animals on the arising of postischaemic heart injury has been only partly investigated. Objective: In our study we tried to assess the influence of subchronical treatment of rats with oestradiol or testosterone on the development of myocardial damage caused by ischaemia/reperfusion. Hypothesis: The sex and sex hormones influence the heart action. After ischaemia/reperfusion less cardiac damage was expected in animals, pretreated with hormones than in control animals. Methods: Experiments were carried out on the isolated hearts of sexually mature rats of both sexes (230-330g). We isolated rat hearts according to Langendorff?s method. The hearts were perfused with Krebs-Henseleit solution aerated with carbogen for 30 min and then subjected to 40 min zero-flow ischaemia followed by 55 min reperfusion. The severity of myocardial damage caused by ischaemia/reperfusion was assessed by postischaemic changes in the following registered parameters: coronary flow, lactate dehydrogenase release rate (LDH), left ventricular pressure (LVP), heart rate and duration of ventricular fibrillation during reperfusion. Results: The coronary flow was higher in the hearts of rats treated with oestradiol or testosterone than in the hearts of control groups. In the hearts of rats treated with oestradiol the postischaemic coronary flow was significantly (p < 0.05) higher than in the hearts of control female group. Postischaemic LDH values were increased in all groups, the highest values were observed in the postgravid group. The difference between postgravid and oestradiol group was statistically significant (p < 0.05). Postischaemnic heart rate values were lower than before ischaemia. The highest values were observed in the postgravid group. Conclusions: In our experiment a protective role of sex hormones on development of ischaemic/reperfusion damage in isolated rat hearts has been shown.