www.medenosrce.net/arhimed   arhimed(a-t>medenosrce.net   [22/04/2026 01:23:54] http://www.medenosrce.net/arhimed/poglej.asp?id=113 Raven nekaterih kazalcev vnetja in inflamatornih citokinov v serumu pacientov s parodontalno boleznijo Avtor: Eva Skaleric Mentor: Prof.dr.Franjo Pikelj Somentor: Asist.mag.Boris Gaspirc 1. IZHODIŠCE: Parodontalna bolezen je najbolj razširjena kronicna vnetna bolezen ljudi. Bolezen povzrocajo mikroorganizmi v zobnih oblogah. Zacetek in razvoj vnetja obzobnih tkiv je pogojen s številom in vrsto mikroorganizmov v plaku ter z vnetno imunološkim odgovorom organizma. Raziskave s pomocjo analiticne epidemiologije in molekularne biologije v zadnjem desetletju so pokazale, da vnetni proces v obzobnih tkivih ne ogroža samo obstoja zob ampak lahko doprinese tudi k razvoju nekaterih sistemskih bolezni. Parodontalna bolezen sodeluje pri razvoju nekaterih bolezni z direktnim vdorom mikroorganizmov preko ulceriranega notranjega gingivalnega epitelija v krvotok in s porastom nekaterih mediatorjev vnetja in inflamatornih citokinov v krvi. 2. NAMEN: Namen raziskave je bil pri sistemsko zdravih krvodajalcih s parodontalno boleznijo ugotoviti raven kazalcev vnetja (CRP, fibrinogen, prokalcitonin) in inflamatornih citokinov (IL-2, sIL-2R, IL-6, IL-8, TNF-?) v njihovem serumu. 3. HIPOTEZA: Predvidevamo, da velikost mehke stene obzobnih žepov v stiku z bakterijami (inficirana parodontalna rana) vpliva na raven vnetnih mediatorjev in citokinov v serumu . 4. METODE: Za oceno inficirane parodontalne rane smo izmerili obseg zobnega vratu na 515 ekstrahiranih zobeh. Povprecne vrednosti obsega zobnega vratu posameznega zoba smo zmnožili s povprecno vrednostjo šestih meritev globine sondiranja posameznega zoba pri preiskovanih osebah. Vsota zmnožkov vrednosti posameznih zob predstavlja velikost inficirane parodontalne rane posameznega pacienta. Vsota zmnožkov meritev globine obzobnih žepov, ki so krvaveli ob sondiranju pa predstavlja velikost aktivne inficirane rane. V raziskavo smo vkljucili 31 sistemsko zdravih moških krvodajalcev starih od 30 do 56 let (povprecno 43,2 leti). Naš parodontalni pregled je vkljuceval meritve globine obzobnih žepov in prisotnosti krvavitve ob sondiranju. Za preverjanje povezanosti med površino aktivne parodontalne rane in koncentracijami vnetnih mediatorjev ter citokinov smo izracunali korelacijske koeficiente po Pearsonu. Za statisticno znacilne smo vzeli P vrednosti, manjše od 0,05. 5. REZULTATI: Rezultati meritev obsega zobnega vratu v zgornji celjusti pri moških so pokazali vrednosti od 20,58 do 32,67 mm, pri ženskah pa od 19,69 do 30,95 mm. V spodnji celjusti pa je znašal obseg zobnega vratu od 17,53 do 32,19 mm, pri ženskah pa od 16,07 do 30,48 mm. Izmerjene velikosti inficirane parodontalne rane so znašale od 4,13 do 27,59 cm2 (v povprecju 17,98 cm2) . Velikosti aktivne inficirane parodontalne rane pa so znašale od 0,86 do 25,4 cm2 ( v povprecju 10,14 cm2). Statisticna analiza je pokazala, da je med vsemi opazovanimi vnetnimi mediatorji in citokini povezava samo med velikostjo inficirane parodontalne rane in IL-2 in njegovim receptorjem.Vrednosti IL-2 in sIL-2R so bile statisticno znacilno višje (p<0,01) pri vecjih skupnih ranah kot tudi pri krvavecih globokih parodontalnih ranah preiskovancev. 6. ZAKLJUCKI: Zakljucki raziskave kažejo, da imajo sistemsko zdravi moški krvodajalci znacilno višje vrednosti IL-2 in sIL-2R v serumu takrat, kadar je prisotna vecja prizadetost obzobnih tkiv izmerjena na osnovi globine obzobnih žepov. Visoka prisotnost krvavecih obzobnih žepov t.i. aktivne inficirane rane dodatno zviša vrednosti IL-2 in sIL-2R v serumu preiskovancev z vnetjem obzobnih tkiv. «» [Abstract / English version] Raven nekaterih kazalcev vnetja in inflamatornih citokinov v serumu pacientov s parodontalno boleznijo Author: Eva Skaleric Mentor: Prof.dr.Franjo Pikelj Co-mentor: Asist.mag.Boris Gaspirc 1. BACKGROUND: Periodontal disease is the most widely spread disease among all chronical inflammatory diseases. The major cause of this disease are microorganisms in dental plaque. The initiation and development of inflammation in periodontal tissue is influenced by the number and type of microorganisms and by inflammatory immune host response. Analytical epidemiology and molecular biology studies in the last decade have shown that periodontal inflammation doesn't only present a risk for tooth loss but may also play a role in the development of some systemic diseases. Periodontal disease plays a role in development of some diseases by direct flow of microorganisms into the bloodstream through ulcerated inner gingival epithelium and by increase of some inflammatory mediators and cytokines in the blood serum. 2. AIM: The aim of our study was to find out the level of inflammatory mediators (CRP, fibrinogen, procalcitonin) and inflammatory citokynes (IL-2,sIL-2R, IL-6, IL-8, TNF-?) in the serum of systemically healthy blood donors with periodontal disease. 3. HYPOTHESIS: Our hypothesis was that the size of soft wall of periodontal pockets in contact with bacteria (infected periodontal wound) influences the level of inflammatory mediators and cytokines in the serum . 4. METHODS: For the evaluation of infected periodontal wound we measured the circumference of the tooth neck at the cemento-enamel junction on 515 extracted teeth. We multiplied the average values of circumferences of tooth necks of individual tooth by the average value of six measurements of probing depth of individual tooth in 31 blood donors. The sum of multiplications of individual teeth presented the size of infected periodontal wound in an individual. The sum of multiplications of measurments of periodontal pockets which were bleeding on probing, presented the size of active infected periodontal wound. In our study we included 31 systemically healthy male blood donors from 30 to 56 years old (in average 43,2 years). Our periodontal examination included pocket depth probing and bleeding on probing. Association between the area of active periodontal wound and the concentration of inflammatory mediators and cytokines was tested with calculating the Pearson's correlation coefficient. A P value of less than 0.05 was considered statistically significant. 5. RESULTS: The results of circumferences of tooth necks in maxilla ranged from 20,58 to 32,67 mm in male population and from 19,69 to 30,95 mm in female population. In mandible the circumferences of tooth necks ranged from 17,53 to 32,19 mm in males and from 16,07 to 30,48 mm in females. The measured sizes of infected periodontal wounds ranged from 4,13 to 27,59 cm2 (in average 17,98 cm2). The measured sizes of active infected periodontal wounds ranged from 0,86 to 25,4 cm2(in average 10,14 cm2). Statistic analysis showed that among all inflammatory mediators and citokynes observed in the serum of blood donors only IL-2 and sIL-2R showed a positive correlation with the size of infected periodontal wound. The levels of IL-2 and sIL-2R in the serum were statistically significantly higher (p < 0,01) with greater size of infected periodontal wound and deep bleeding periodontal wound. 6. CONCLUSIONS: The results of our study show a significantly higher level of serum IL-2 and sIL-2R in systemically healthy male blood donors when a greater periodontal tissue destruction is present. If a large number of bleeding periodontal pockets (active infected periodontal wound) is additionally present, the levels of IL-2 and sIL-2R in serum are even higher.